We are currently offering a fantastic career opportunity for a highly motivated and experienced antibody discovery/development scientist to join Antharis Therapeutics, a pre-clinical company based in San Diego. The company is committed to discovering and developing novel immunotherapeutics (monoclonal antibodies and antibody-drug conjugates) for an array of disease states. The approaches for antibody discovery, including hybridoma technology, aim to streamline the development of lead candidates for entering the clinic in an expedited fashion.
We are seeking a Scientist I with hands-on experience in antibody discovery using hybridoma technology. Responsibilities will include designing and executing immunization and screening strategies to generate panels of antibodies that target various proteins in the oncology and immune-oncology fields. The candidate must have in-depth knowledge of current state-of-the-art antibody generation technologies and be able to apply this to practice at the bench.
• Design and execute on approaches to immunizemice and perform electrofusions
• Design and execute hybridoma cell cultureusing both liquid and semi-solid media
• Design and execute screening protocols usingboth ELISA and flow cytometry methods
• Proactively propose strategies to projectteams to generate the target antibodies
• Execute on all stages of antibody generationto deliver diverse panels of antibodies meeting the required criteria.
• Integrate data from multiple sources (binding,epitope, sequences, and protein characterization) to generate a complete datapackage for antibody characterization.
• Maintain detailed records of all experimentsin ELN and using in-house databases and tracking systems.
• Direct a group of scientists abroad tofacilitate the hybridoma generation work flow
• Responsible for delivering both data analysispackages as well as purified antibodies to project teams
• Trouble-shoot and optimize protocols andpropose creative solutions to improve efficiency to existing processes.
• Proactively evaluate new technologies,instrumentation, and assays available from the literature or scientificmeetings to improve in-house platforms.
▫ Implement and routinely run disease-relevant in-vitro and in-vivo teststo probe for antibody therapeutic efficacy.
▫ Coordinate and carry-out in-vitro and in-vivo tests to probe assetefficacy in relevant in-vitro and in-vivo disease models.
▫ Plan and execute mechanism of action studies on the Company’s assetpipeline.
▫ Ensure rapid and high-quality project advancement.
▫ Responsible for day-to-day bench operations and leadership over eachproject inflection points, including: target identification and discussions,developing project plans, carrying out experiments from conception to assetdevelopment and testing, and controlling project execution to ensure completionof projects to scope, schedule and budget.
▫ Responsible for ensuring that project risks, both technical andmanagerial, are identified and appropriately addressed, as well as develop riskmitigation plans.
▫ Regularly assess and report the status of the projects directly to theCSO and the VP of Research & Development.
▫ Drive day-to-day project activities to achieve results including solvingtechnical and other project challenges, managing resource conflicts, breakingdown barriers to meet product development deadlines, and company goals andobjectives.
• Ph.D. and postdoctoral in Molecular and CellBiology, Biochemistry, or advanced degree with 5+ years of relevant experience.
• Demonstrated experience successfully executingantibody generation using hybridoma approaches to identify antibody panels isrequired.
• Extensive experience with antibody bindingassays and analysis, especially ELISA, biosensor, and multi-color flowcytometry is required.
• Experience with PCR, molecular cloning, andtransfection of mammalian cells.
• Strong experience in Excel, Graphpad Prism,flow cytometry analysis software is required
• Strong focus, motivation, and commitment tohands-on lab work is required.
• Excellent organization and communicationskills.
• Ability to multi-task in a fast-pacedenvironment.
• Ability to work and thrive in a collaborative,cross-functional team-oriented
▫ Legally authorized to work in the US.
